NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. In April 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       


Contents

Summary

10x buffer for PCR with Pfu. Makes 10mL.

Ingredients

  • 2 mL 1M Tris-HCl pH 8.8
  • 333 μL 3M KCl
  • 500 μL 20% Triton X-100
  • 1 mL 100x BSA
  • 200 μL 1M MgSO4
  • 5.967 mL H2O

Protocol

  1. Mix, vortex.
  2. Store at -20°C

Notes

  • We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -Jkm 18:18, 2 August 2006 (EDT)
  • Should be supplemented with MgCl2 when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works for most applications.

Source

Unknown. Ask Kristy.

Contact

Kristy