NOTICE: You are viewing a page of the openwetware wiki. Our "dewikify" feature makes a wiki page appear as a normal web page. In April 2017, this feature will GO AWAY and this URL will redirect to the source URL on our wiki. We're sorry for the inconvenience.

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       



dNTP mixture: 40 mM NTPs, 10 mM each. Makes 1.25mL for a total of 25, 50 μL aliquots.


  • 100 mM ATP
  • 100 mM CTP
  • 100 mM GTP
  • 100 mM TTP
  • dH2O, preferably DEPC treated


  1. Add 125 μL each NTP.
  2. Add 750 μL dH2O.
  3. Mix, Vortex.
  4. Aliquot out 50 μL of mixture into 0.6 mL microfuge tubes.


Final concentrations based on NEB dNTP pre-mixed solution. Additional questions, ask Midori.